5H7T
A GH19 chitinase domain from the Cryptomeria japnonica pollen (CJP-4) allergen
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-01-23 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.011, 74.304, 35.811 |
| Unit cell angles | 90.00, 99.88, 90.00 |
Refinement procedure
| Resolution | 37.150 - 1.190 |
| R-factor | 0.14733 |
| Rwork | 0.146 |
| R-free | 0.16375 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.001 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 37.150 |
| High resolution limit [Å] | 1.190 |
| Number of reflections | 51012 |
| <I/σ(I)> | 20.5 |
| Completeness [%] | 94.1 |
| Redundancy | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M BIS-TRIS pH 6.5 15 % PEG 3350 |
