5H7N
Crystal structure of human NLRP12-PYD with a MBP tag
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-22 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.540, 104.550, 110.940 |
| Unit cell angles | 90.00, 98.95, 90.00 |
Refinement procedure
| Resolution | 47.182 - 1.849 |
| R-factor | 0.1806 |
| Rwork | 0.179 |
| R-free | 0.22370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ifp |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.310 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2481: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.900 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 161270 | |
| <I/σ(I)> | 17.3 | 1.7 |
| Completeness [%] | 98.6 | 96 |
| Redundancy | 5.7 | 5.6 |
| CC(1/2) | 0.998 | 0.636 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 30% PEG 550, 0.2M Ammonium Sulfate, NaAc 4.6 |
