5H7L
Complex of Elongation factor 2-50S ribosomal protein L12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2012-11-17 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 79.774, 121.902, 199.265 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.432 - 3.100 |
| R-factor | 0.2329 |
| Rwork | 0.230 |
| R-free | 0.28540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.644 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.270 |
| High resolution limit [Å] | 3.090 | 3.090 |
| Rmerge | 0.070 | 0.598 |
| Number of reflections | 36125 | |
| <I/σ(I)> | 15.69 | 2.86 |
| Completeness [%] | 99.0 | 98 |
| Redundancy | 5.75 | 5.72 |
| CC(1/2) | 0.999 | 0.868 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 6 | 293 | 10% PEG10000, 0.1 M MES, 0.5 M Lithium acetate dehydrate |
