5H7C

Crystal structure of a repeat protein with two Protein A-DHR14 repeat modules

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	80
Detector technology	CCD
Collection date	2016-06-29
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97934
Spacegroup name	P 1
Unit cell lengths	48.658, 75.528, 80.013
Unit cell angles	84.19, 83.72, 75.21

Refinement procedure

Resolution	29.052 - 2.700
R-factor	0.2055
Rwork	0.203
R-free	0.26220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4znc 5cwh
RMSD bond length	0.003
RMSD bond angle	0.677
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.700	2.700
Number of reflections	30168
<I/σ(I)>	15.2
Completeness [%]	95.9	94.6
Redundancy	1.8	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	296	0.1M Tris pH 8.0, 33.75%(w/v) PEG MME 2000