5H7A

Crystal structure of a repeat protein with four Protein A repeat module

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	80
Detector technology	CCD
Collection date	2016-04-20
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97934
Spacegroup name	P 1
Unit cell lengths	55.406, 80.804, 130.334
Unit cell angles	89.83, 89.62, 90.12

Refinement procedure

Resolution	34.297 - 2.700
R-factor	0.2473
Rwork	0.246
R-free	0.28460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4znc 5coc
RMSD bond length	0.004
RMSD bond angle	0.822
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.700	2.700
Number of reflections	65235
<I/σ(I)>	8.2	2.24
Completeness [%]	97.0	94.2
Redundancy	1.9	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.5	296	0.1M sodium acetate pH 4.5, 43.2% (w/v) PEG 1000