5H79
Crystal structure of a repeat protein with three Protein A repeat module
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2016-04-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.689, 46.614, 74.311 |
| Unit cell angles | 90.00, 107.29, 90.00 |
Refinement procedure
| Resolution | 28.018 - 2.700 |
| R-factor | 0.2321 |
| Rwork | 0.230 |
| R-free | 0.27780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4znc 5coc |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.630 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 10513 | |
| <I/σ(I)> | 15.5 | 2.14 |
| Completeness [%] | 98.7 | 98.6 |
| Redundancy | 3.3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.5 | 296 | 0.1M sodium acetate pH 4.5, 13.5%(w/v) PEG 1000 |
