5H76

Crystal structure of the DARPin-Protein A fusion protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	80
Detector technology	CCD
Collection date	2015-10-22
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97934
Spacegroup name	P 1 21 1
Unit cell lengths	70.753, 94.684, 70.916
Unit cell angles	90.00, 117.29, 90.00

Refinement procedure

Resolution	33.172 - 2.600
R-factor	0.219
Rwork	0.216
R-free	0.24990
RMSD bond length	0.003
RMSD bond angle	0.563
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.690
High resolution limit [Å]	2.600	2.600
Number of reflections	27769
<I/σ(I)>	9.9	2.22
Completeness [%]	99.6	99.6
Redundancy	3.4	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	9.5	296	0.63M sodium potassium phosphate pH 9.5