5H72
Structure of the periplasmic domain of FliP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-02 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 114.880, 114.880, 193.781 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.923 - 2.400 |
| R-factor | 0.2138 |
| Rwork | 0.211 |
| R-free | 0.26060 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.859 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX (1.9_1692) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.600 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.088 | 0.357 |
| Number of reflections | 30262 | |
| <I/σ(I)> | 13.5 | 5.5 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.1 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.4 | 277 | 0.1M phosphate-citrate pH 4.4, 36% MPD |
