5H71
Structure of alginate-binding protein AlgQ2 in complex with an alginate trisaccharide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-10-09 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 76.274, 53.150, 127.451 |
Unit cell angles | 90.00, 93.63, 90.00 |
Refinement procedure
Resolution | 29.852 - 1.549 |
R-factor | 0.1686 |
Rwork | 0.167 |
R-free | 0.19650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1j1n |
RMSD bond length | 0.007 |
RMSD bond angle | 1.126 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.580 |
High resolution limit [Å] | 1.549 | 1.549 |
Rmerge | 0.084 | 0.420 |
Number of reflections | 143400 | |
<I/σ(I)> | 25.9 | 3.2 |
Completeness [%] | 96.8 | 89.1 |
Redundancy | 3.8 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293.15 | 30% PEG4000, 0.2 M lithium sulfate, 0.1 M tris(hydroxymethyl)aminomethan-hydrochloride acid |