5H6Y

The BPTF Bromodomain Recognising H4K12Cr peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.987
Spacegroup name	P 21 21 2
Unit cell lengths	66.350, 71.186, 27.255
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.268 - 2.000
R-factor	0.1885
Rwork	0.187
R-free	0.22370
RMSD bond length	0.007
RMSD bond angle	0.984
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	2.000
Number of reflections	9284
<I/σ(I)>	13.93
Completeness [%]	99.4
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	289	1 M succinic acid pH 7.0, 0.1 M HEPES pH 7.0, 1% PEG MME 2000