5H6X
The crystal structure of RpoS fragment including a partial region 1.2 and region 2 from intracellular pathogen Legionella pneumophila
Experimental procedure
| Experimental method | LAUE |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97907 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.910, 68.905, 70.357 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.600 |
| R-factor | 0.16578 |
| Rwork | 0.164 |
| R-free | 0.19218 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4yg2 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.303 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.088 | 0.500 |
| Number of reflections | 25054 | |
| <I/σ(I)> | 31.6 | 6.1 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 12.7 | 13.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 285 | 12% PEG8000 0.2M NaCl 0.1M Hepes pH7.5 |
