5H6W
Structure of acetyl-Coenzyme A synthase Alpha subunit C-terminal domain F598H mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-09-17 |
| Detector | RIGAKU |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.512, 86.601, 86.762 |
| Unit cell angles | 90.00, 92.15, 90.00 |
Refinement procedure
| Resolution | 43.340 - 2.200 |
| R-factor | 0.17568 |
| Rwork | 0.173 |
| R-free | 0.22803 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3s2x |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.849 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.564 | |
| Number of reflections | 24711 | |
| <I/σ(I)> | 15.76 | 2.56 |
| Completeness [%] | 99.6 | 99.6 |
| Redundancy | 3.3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 0.2 M Citrate Sodium PH4.6, 1.6 M Sodium phosphate monobasic, 0.4 M Potassium phosphate dibasic |
