5H6T
Crystal structure of Hydrazidase from Microbacterium sp. strain HM58-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2012-11-11 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 122.162, 128.068, 159.304 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.970 - 1.600 |
| R-factor | 0.16551 |
| Rwork | 0.165 |
| R-free | 0.17809 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3h0l |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.390 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MoRDa |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.054 | 0.232 |
| Number of reflections | 163385 | |
| <I/σ(I)> | 47.2 | 8.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.1 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1 M imidazole, 0.17 M LiSO4, 2 M (NH4)2SO4 |
