5H6O

Porphobilinogen deaminase from Vibrio Cholerae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2016-02-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	I 41 2 2
Unit cell lengths	94.230, 94.230, 165.340
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.125 - 2.702
R-factor	0.2382
Rwork	0.236
R-free	0.27660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gtk
RMSD bond length	0.002
RMSD bond angle	0.680
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	47.570
High resolution limit [Å]	2.700
Number of reflections	10562
<I/σ(I)>	38.82
Completeness [%]	99.9
Redundancy	14.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	20% (w/v) PEG 3350, 100 mM Magnesium Nitrate