5H6M
DNA targeting ADP-ribosyltransferase Pierisin-1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2008-12-20 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 134.720, 134.720, 73.120 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.542 - 1.900 |
R-factor | 0.20377 |
Rwork | 0.203 |
R-free | 0.21940 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.147 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.800 |
Number of reflections | 70003 |
<I/σ(I)> | 36.18 |
Completeness [%] | 98.8 |
Redundancy | 9.89 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.1M Tris-HCl pH8.0, 20% (v/v) MPD |