5H6L

DNA targeting ADP-ribosyltransferase Pierisin-1 in complex with beta-NAD+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	93
Detector technology	CCD
Collection date	2009-01-19
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0000
Spacegroup name	P 32 2 1
Unit cell lengths	134.744, 134.744, 73.388
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	9.999 - 2.100
R-factor	0.24337
Rwork	0.243
R-free	0.25949
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.031
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall
Low resolution limit [Å]	45.670
High resolution limit [Å]	2.100
Number of reflections	45134
<I/σ(I)>	13.5
Completeness [%]	99.6
Redundancy	10.33

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	0.1M Tris-HCl pH8.0, 20% (v/v) MPD