5H6H
Crystal Structure of Hyperthermophilic Thermotoga maritima L-Ribulose 3-Epimerase with Mn2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-30 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97960 |
| Spacegroup name | P 1 |
| Unit cell lengths | 50.318, 55.275, 58.699 |
| Unit cell angles | 107.22, 102.71, 91.75 |
Refinement procedure
| Resolution | 21.581 - 1.446 |
| R-factor | 0.1752 |
| Rwork | 0.174 |
| R-free | 0.19930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zvr |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.855 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.480 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.050 | 0.323 |
| Number of reflections | 98787 | |
| <I/σ(I)> | 25.79 | 4.04 |
| Completeness [%] | 94.5 | 93.8 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 100mM MES, 5%(w/v) PEG1000, 20%(v/v) PEG200 |
