5H6B

Crystal structure of a thermostable lipase from Marine Streptomyces

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-06
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.2822
Spacegroup name	P 64
Unit cell lengths	90.730, 90.730, 70.620
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	70.630 - 2.300
R-factor	0.1796
Rwork	0.178
R-free	0.20580
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.360
Data reduction software	MOSFLM
Data scaling software	Aimless (0.5.15)
Phasing software	PHASES
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.630	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.106	0.718
Number of reflections	14806
<I/σ(I)>	16.2
Completeness [%]	100.0	100
Redundancy	10.8	10.9
CC(1/2)	0.998	0.903

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	293	0.2M Zn(AC)2, 0.1M imidazole, pH 6.5, 10% PEG 8000