5H69
Crystal structure of an asymmetric dimer of the Geobacillus stearothermophilus SMC hinge domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-01 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 41 |
| Unit cell lengths | 77.144, 77.144, 114.795 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.563 - 2.200 |
| R-factor | 0.195743440621 |
| Rwork | 0.193 |
| R-free | 0.24074 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gxj |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.846 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MrBUMP |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.065 | 0.214 |
| Number of reflections | 34064 | |
| <I/σ(I)> | 18 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.1 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M magnesium formate, 15%(w/v) PEG 33500 |
