5H5T

Crystal structure of the flagellar cap protein FliD D2-D3 domains from Salmonella Typhimurium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2016-10-06
Detector	ADSC QUANTUM 270
Wavelength(s)	1.00004
Spacegroup name	P 21 21 21
Unit cell lengths	78.688, 106.062, 141.704
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.500
R-factor	0.23144
Rwork	0.229
R-free	0.27847
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5h5v
RMSD bond length	0.009
RMSD bond angle	1.115
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.540
High resolution limit [Å]	2.500	2.500
Rmerge	0.084
Number of reflections	40695
<I/σ(I)>	20.2
Completeness [%]	97.4
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	16% PEG 1000, 0.1 M sodium acetate pH 3.5, 0.2 M zinc acetate