5H5P

Crystal structure of Myelin-gene Regulatory Factor DNA binding domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-07
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 3 2 1
Unit cell lengths	103.970, 103.970, 46.730
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.032 - 2.461
R-factor	0.1796
Rwork	0.175
R-free	0.22390
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.209
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	44.950
High resolution limit [Å]	2.366
Number of reflections	24100
<I/σ(I)>	8.52
Completeness [%]	99.8
Redundancy	8.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	Tris, Magnesium acetate, PEG4000