5H5H

Staphylococcus aureus FtsZ-GDP R29A mutant in T state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-16
Detector	RAYONIX MX300HE
Wavelength(s)	0.900
Spacegroup name	C 1 2 1
Unit cell lengths	71.525, 50.834, 88.477
Unit cell angles	90.00, 110.63, 90.00

Refinement procedure

Resolution	41.400 - 1.700
R-factor	0.184
Rwork	0.182
R-free	0.22600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3voa
RMSD bond length	0.015
RMSD bond angle	1.707
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.730
High resolution limit [Å]	1.700	4.610	1.700
Rmerge	0.130	0.084	0.960
Rmeas		0.097	1.108
Rpim		0.049	0.549
Number of reflections	32530
<I/σ(I)>	4.9
Completeness [%]	99.5	93.3	100
Redundancy	4.1	3.9	4.1
CC(1/2)		0.986	0.611

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.1	293	100mM Tris, 43% w/v PEP629, 300mM KCl