5H5A
Mdm12 from K. lactis (1-239), Lys residues are uniformly dimethyl modified
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-10-26 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1 |
Spacegroup name | P 1 |
Unit cell lengths | 42.868, 48.524, 130.126 |
Unit cell angles | 90.01, 91.50, 90.01 |
Refinement procedure
Resolution | 50.000 - 2.260 |
R-factor | 0.25248 |
Rwork | 0.251 |
R-free | 0.28615 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 2.003 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.250 |
Number of reflections | 527803 |
<I/σ(I)> | 11.3 |
Completeness [%] | 97.1 |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.26M NaH2PO4, 0.14M K2HPO4 pH 5.6 |