5H58
Structural and dynamics studies of the TetR family protein, CprB from Streptomyces coelicolor in complex with its biological operator sequence
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-11-28 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.9737 |
Spacegroup name | P 32 |
Unit cell lengths | 149.810, 149.810, 70.460 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.249 - 3.991 |
R-factor | 0.2215 |
Rwork | 0.217 |
R-free | 0.28160 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.559 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 3.950 |
Number of reflections | 15346 |
<I/σ(I)> | 22.4 |
Completeness [%] | 99.7 |
Redundancy | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293.15 | 0.1 M ammonium acetate, 0.02 M MgCl2.6H2O, 0.05 M HEPES sodium, 5% PEG 8000 |