5H4Z
Crystal structure of S202G mutant of human SYT-5 C2A domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-06-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97916 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 76.005, 118.656, 109.464 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.010 |
| R-factor | 0.20254 |
| Rwork | 0.200 |
| R-free | 0.25745 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5h4y |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.331 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.167 | 0.837 |
| Number of reflections | 9990 | |
| <I/σ(I)> | 15.6 | 3.1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 287 | 0.1M calcium acetate, 0.1M sodium acetate pH 4.0, 10%(w/v) PEG4000 |
