5H4Y
Crystal structure of human synaptotagmin 5 C2A domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | Cu FINE FOCUS |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-11-15 |
| Detector | RIGAKU |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 65 |
| Unit cell lengths | 93.966, 93.966, 28.052 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.760 - 1.900 |
| R-factor | 0.17259 |
| Rwork | 0.171 |
| R-free | 0.21161 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.256 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.079 | 0.425 |
| Number of reflections | 11445 | |
| <I/σ(I)> | 33.9 | 5.7 |
| Completeness [%] | 99.9 | 99.1 |
| Redundancy | 12.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 284 | 0.1M calcium acetate, 0.1M sodium acetate pH 5.6, 12.5%(w/v) PEG4000 |
