5H4U
Crystal structure of cellulase from Antarctic springtail, Cryptopygus antarcticus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-14 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 31 |
| Unit cell lengths | 81.714, 81.714, 89.352 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.857 - 2.600 |
| R-factor | 0.2256 |
| Rwork | 0.219 |
| R-free | 0.28910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oa9 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.388 |
| Data scaling software | HKL-2000 |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 21047 | |
| <I/σ(I)> | 16.1 | |
| Completeness [%] | 97.8 | |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 4.2 | 295 | 20% PEG 8K, 0.1M phosphate-citrate pH 4.2, 0.2M sodium chloride |
