5H4S
Crystal structure of a rhamnose-binding lectin SUL-I from the toxopneustid sea urchin Toxopneustes pileolus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-03-03 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 101.760, 44.210, 71.840 |
| Unit cell angles | 90.00, 124.08, 90.00 |
Refinement procedure
| Resolution | 29.750 - 1.800 |
| R-factor | 0.15906 |
| Rwork | 0.157 |
| R-free | 0.20523 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zx2 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.897 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 29.800 |
| High resolution limit [Å] | 1.800 |
| Number of reflections | 24749 |
| <I/σ(I)> | 20.1 |
| Completeness [%] | 99.8 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.2M Lithium sulfate, 0.1M Phosphate-citrate (pH4.2), 20% PEG 1000, 0.05% DDM |
