5H4H
Structure of PIN-domain protein (VapC4 toxin) from Pyrococcus horikoshii determined at 2.2 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU ULTRAX 18 |
Temperature [K] | 300 |
Detector technology | CCD |
Collection date | 2011-01-10 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.54179 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 51.970, 45.260, 53.620 |
Unit cell angles | 90.00, 104.75, 90.00 |
Refinement procedure
Resolution | 51.850 - 2.230 |
R-factor | 0.21012 |
Rwork | 0.207 |
R-free | 0.25904 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1v96 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.236 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.850 | 2.290 |
High resolution limit [Å] | 2.210 | 2.210 |
Rmerge | 0.420 | |
Number of reflections | 11886 | |
<I/σ(I)> | 8.5 | 2.4 |
Completeness [%] | 97.2 | 71.2 |
Redundancy | 3.3 | 3 |
CC(1/2) | 0.540 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 0.2M Sodium acetate trihydrate, 0.1M Tris-HCl, 30%(w/v) PEG 4000 |