5H4H
Structure of PIN-domain protein (VapC4 toxin) from Pyrococcus horikoshii determined at 2.2 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU ULTRAX 18 |
| Temperature [K] | 300 |
| Detector technology | CCD |
| Collection date | 2011-01-10 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.54179 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.970, 45.260, 53.620 |
| Unit cell angles | 90.00, 104.75, 90.00 |
Refinement procedure
| Resolution | 51.850 - 2.230 |
| R-factor | 0.21012 |
| Rwork | 0.207 |
| R-free | 0.25904 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1v96 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.236 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.850 | 2.290 |
| High resolution limit [Å] | 2.210 | 2.210 |
| Rmerge | 0.420 | |
| Number of reflections | 11886 | |
| <I/σ(I)> | 8.5 | 2.4 |
| Completeness [%] | 97.2 | 71.2 |
| Redundancy | 3.3 | 3 |
| CC(1/2) | 0.540 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 0.2M Sodium acetate trihydrate, 0.1M Tris-HCl, 30%(w/v) PEG 4000 |
