5H46
Mycobacterium smegmatis Dps1 mutant - F47E
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-08-05 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 175.070, 175.070, 175.070 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.147 - 2.850 |
| R-factor | 0.23194 |
| Rwork | 0.229 |
| R-free | 0.26025 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vei |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.430 |
| Data reduction software | REFMAC |
| Data scaling software | REFMAC |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.147 | 3.160 |
| High resolution limit [Å] | 2.850 | 3.000 |
| Number of reflections | 5200 | |
| <I/σ(I)> | 30.3 | |
| Completeness [%] | 99.9 | |
| Redundancy | 42.7 | |
| CC(1/2) | 0.680 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7.5 | 293 | 100 mM Na-HEPES, 200 mM CaCl2, 10% PEG 550 |
