5H41
Crystal Structure of 1,2-beta-oligoglucan phosphorylase from Lachnoclostridium phytofermentans in complex with sophorose, isofagomine, sulfate ion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-11-28 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.692, 94.877, 157.357 |
| Unit cell angles | 90.00, 100.81, 90.00 |
Refinement procedure
| Resolution | 48.270 - 2.000 |
| R-factor | 0.16954 |
| Rwork | 0.168 |
| R-free | 0.20406 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5h3z |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.704 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.127 | 0.502 |
| Number of reflections | 168440 | |
| <I/σ(I)> | 3.7 | 1.6 |
| Completeness [%] | 96.0 | 99 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | PEG3350, calcium acetate, Tris-HCl |
