5H40
Crystal Structure of 1,2-beta-oligoglucan phosphorylase from Lachnoclostridium phytofermentans in complex with sophorose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-21 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.780, 94.330, 156.920 |
| Unit cell angles | 90.00, 97.60, 90.00 |
Refinement procedure
| Resolution | 86.020 - 2.200 |
| R-factor | 0.18098 |
| Rwork | 0.179 |
| R-free | 0.21139 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5h3z |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.673 |
| Data reduction software | iMOSFLM |
| Data scaling software | iMOSFLM |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.020 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.070 | 0.136 |
| Number of reflections | 123410 | |
| <I/σ(I)> | 9.3 | 4.3 |
| Completeness [%] | 96.9 | 91.1 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | PEG3350, Tris-HCl, calcium acetate |
