5H3X
The structure of the N-terminal of the fibronectin/fibrinogen-binding protein from Streptococcus suis (FBPS)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BSRF BEAMLINE 1W2B |
Synchrotron site | BSRF |
Beamline | 1W2B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-03-03 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.0 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 70.555, 70.555, 110.183 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.597 - 2.100 |
R-factor | 0.2175 |
Rwork | 0.216 |
R-free | 0.24550 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.215 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.5_2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.084 | 0.512 |
Number of reflections | 16622 | |
<I/σ(I)> | 29.98 | |
Completeness [%] | 97.6 | |
Redundancy | 12.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1M Na Citrate (pH 5.6), 20%(w/v) PEG 4,000, 0.2M NaCl |