5H3W
The structure of the C-terminal of the fibronectin/fibrinogen-binding protein from Streptococcus suis (FBPS)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.338, 103.645, 83.278 |
| Unit cell angles | 90.00, 92.19, 90.00 |
Refinement procedure
| Resolution | 38.613 - 2.600 |
| R-factor | 0.2207 |
| Rwork | 0.219 |
| R-free | 0.24870 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.315 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-2000 |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.115 | 0.338 |
| Number of reflections | 21058 | |
| <I/σ(I)> | 17.57 | |
| Completeness [%] | 97.1 | |
| Redundancy | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1M HEPES (pH 7.5), 10%(w/v) PEG 4,000, 500mM NaCl |
