5H3V
Crystal structure of a Type IV Secretion System Component CagX in Helicobacter pylori
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AichiSR BEAMLINE BL2S1 |
| Synchrotron site | AichiSR |
| Beamline | BL2S1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.080, 61.620, 48.420 |
| Unit cell angles | 90.00, 90.23, 90.00 |
Refinement procedure
| Resolution | 38.070 - 1.400 |
| R-factor | 0.20255 |
| Rwork | 0.200 |
| R-free | 0.24887 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3jqo |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.712 |
| Data reduction software | HKL-2000 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.070 | 1.440 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Number of reflections | 34978 | 1587 |
| <I/σ(I)> | 12.3 | 3 |
| Completeness [%] | 91.7 | 56 |
| Redundancy | 3.7 | 3.4 |
| CC(1/2) | 0.997 | 0.803 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.1M Tris-HCl, 27% PEG4K, 10% isopropanol |
