5H3J
Crystal structure of Grasp domain of Grasp55 complexed with the Golgin45 C-terminus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-06-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97368 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 97.681, 35.855, 64.030 |
| Unit cell angles | 90.00, 93.54, 90.00 |
Refinement procedure
| Resolution | 31.954 - 1.330 |
| R-factor | 0.1379 |
| Rwork | 0.137 |
| R-free | 0.16230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rle |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.235 |
| Data reduction software | xia2 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.650 | 1.295 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Number of reflections | 122914 | |
| <I/σ(I)> | 8.38 | |
| Completeness [%] | 99.8 | |
| Redundancy | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | PEG4000, potassium sodium tartrate, Tris |
