5H35
Crystal structures of the TRIC trimeric intracellular cation channel orthologue from Sulfolobus solfataricus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 102.734, 171.391, 103.265 |
| Unit cell angles | 90.00, 117.94, 90.00 |
Refinement procedure
| Resolution | 49.404 - 2.642 |
| R-factor | 0.2127 |
| Rwork | 0.211 |
| R-free | 0.25200 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.350 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.640 |
| Number of reflections | 90876 |
| <I/σ(I)> | 14.59 |
| Completeness [%] | 98.2 |
| Redundancy | 7.02 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | PEG4000, MgCl2, NaCl, Tris-HCl pH 8.5 |
