5H2X

Crystal structure of the karyopherin Kap60p bound to the SUMO protease Ulp1p (150-172)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-14
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	54.570, 77.480, 114.590
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.268 - 2.200
R-factor	0.203
Rwork	0.202
R-free	0.22840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5h2w
RMSD bond length	0.003
RMSD bond angle	0.738
Data reduction software	iMOSFLM
Data scaling software	Aimless (0.5.8)
Phasing software	MOLREP
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.270	49.270	2.270
High resolution limit [Å]	2.200	9.070	2.200
Rmerge	0.146	0.038	0.787
Rmeas	0.160
Rpim	0.065
Total number of observations	149220
Number of reflections	25386
<I/σ(I)>	9.9
Completeness [%]	100.0	99.6	100
Redundancy	5.9	4.8	6.1
CC(1/2)	0.995	0.998	0.777

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1M sodium phosphate, 12% PEG8000