5H2V

Crystal structure of the karyopherin Kap121p bound to the SUMO protease Ulp1p

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2012-12-04
Detector	RAYONIX MX-225
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	76.830, 124.710, 130.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.713 - 2.800
R-factor	0.2001
Rwork	0.198
R-free	0.24120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3w3w
RMSD bond length	0.005
RMSD bond angle	0.889
Data reduction software	iMOSFLM
Data scaling software	Aimless (0.1.27)
Phasing software	MOLREP
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	36.710	36.710	2.950
High resolution limit [Å]	2.800	8.850	2.800
Rmerge	0.167	0.050	0.728
Rmeas	0.180
Rpim	0.067
Total number of observations	224445
Number of reflections	31520
<I/σ(I)>	8.4
Completeness [%]	100.0	98.7	100
Redundancy	7.1	6.4	7.2
CC(1/2)	0.995	0.997	0.778

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.1M HEPES, 10% 2-propanol, 24% PEG20000