5H2T
Structure of trehalose synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-11 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97776 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 248.760, 133.116, 211.777 |
| Unit cell angles | 90.00, 112.54, 90.00 |
Refinement procedure
| Resolution | 43.567 - 2.796 |
| R-factor | 0.2076 |
| Rwork | 0.205 |
| R-free | 0.25190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.474 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Refinement software | PHENIX (1.10.1-2155) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 43.570 |
| High resolution limit [Å] | 2.796 |
| Number of reflections | 156232 |
| <I/σ(I)> | 14 |
| Completeness [%] | 99.0 |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291.15 | 1M Ammonium sulfate, 0.1M Bis-Tris PH 5.5, 1% PEG 3350 |
