5H2N

A three dimensional movie of structural changes in bacteriorhodopsin: structure obtained 95.2 us after photoexcitation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	SACLA BEAMLINE BL3
Synchrotron site	SACLA
Beamline	BL3
Temperature [K]	293
Detector technology	CCD
Collection date	2016-02-05
Detector	MPCCD
Wavelength(s)	1.63
Spacegroup name	P 63
Unit cell lengths	62.500, 62.500, 112.000
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	21.912 - 2.100
R-factor	0.1574
Rwork	0.156
R-free	0.17870
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5b6v
RMSD bond length	0.003
RMSD bond angle	0.819
Data reduction software	CrystFEL (0.6.2)
Data scaling software	CrystFEL (0.6.2)
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	38.920
High resolution limit [Å]	2.100
Number of reflections	14513
<I/σ(I)>	9.27
Completeness [%]	100.0
Redundancy	279
CC(1/2)	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	5.4	293	0.1M Na/K phosphate pH5.4, 30% PEG 2000