5H2F

Crystal structure of the PsbM-deletion mutant of photosystem II

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-12-13
Detector	ADSC QUANTUM 210
Wavelength(s)	0.8
Spacegroup name	P 21 21 21
Unit cell lengths	121.954, 226.993, 285.886
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.200
R-factor	0.177
Rwork	0.174
R-free	0.22600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3wu2
RMSD bond length	0.016
RMSD bond angle	2.335
Data reduction software	XSCALE
Data scaling software	SCALA (3.3.22)
Phasing software	MOLREP
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	46.794	2.320
High resolution limit [Å]	2.200	6.960	2.200
Rmerge		0.029	0.705
Rmeas	0.092	0.032	0.788
Rpim	0.039	0.014	0.344
Total number of observations	2003997	62052	272075
Number of reflections	385629
<I/σ(I)>	13.6	48.7	2.2
Completeness [%]	96.7	91.8	96.6
Redundancy	5.2	5.1	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6.1	285	PEG1450, sodium chloride, calcium chloride, magnesium sulfate