5H2A

Crystal structure of Osh1 ANK domain from Kluyveromyces lactis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-03
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97950
Spacegroup name	P 21 21 2
Unit cell lengths	91.463, 189.092, 41.337
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.270 - 1.900
R-factor	0.1805
Rwork	0.179
R-free	0.21480
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.806
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.930
High resolution limit [Å]	1.900	1.900
Rmerge	0.075	0.460
Number of reflections	57626
<I/σ(I)>	47	9.7
Completeness [%]	99.9	100
Redundancy	9.5	9.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	290	Bicine-HCl pH 9.0, 20% PEG 3350, 0.2M MgSO4