5H29
Crystal Structure of the NTD_N/C domain of Alkylhydroperoxide Reductase AhpF from Enterococcus Faecalis (V583)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-10-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.00 |
Spacegroup name | P 41 3 2 |
Unit cell lengths | 134.278, 134.278, 134.278 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.320 - 2.300 |
R-factor | 0.19558 |
Rwork | 0.194 |
R-free | 0.22871 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1zyn |
RMSD bond length | 0.005 |
RMSD bond angle | 0.947 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.051 | 0.660 |
Number of reflections | 19025 | |
<I/σ(I)> | 22.1 | 3 |
Completeness [%] | 99.8 | 100 |
Redundancy | 11.4 | 11.7 |
CC(1/2) | 0.990 | 0.880 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.1 M Bis-Tris (pH 6.5), 2.0 M ammonium sulfate and 0.1 M sodium chloride |