5H28
Crystal structure of Osh1 ANK domain from Saccharomyces cerevisia
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-07-05 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.97950 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 29.465, 72.202, 49.008 |
| Unit cell angles | 90.00, 93.53, 90.00 |
Refinement procedure
| Resolution | 24.550 - 1.500 |
| R-factor | 0.184 |
| Rwork | 0.184 |
| R-free | 0.21400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.062 | 0.371 |
| Number of reflections | 80061 | |
| <I/σ(I)> | 31.6 | 4.8 |
| Completeness [%] | 99.6 | 99 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 0.1M HEPES-NaOH pH 7.0, 20% PEG 1500 |
