5H23

Crystal structure of Chikungunya virus capsid protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR-H
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2015-08-25
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.54
Spacegroup name	P 1
Unit cell lengths	33.307, 39.096, 59.261
Unit cell angles	92.36, 91.18, 103.00

Refinement procedure

Resolution	32.713 - 2.200
R-factor	0.1801
Rwork	0.176
R-free	0.26070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vcp
RMSD bond length	0.007
RMSD bond angle	1.089
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.061	0.221
Number of reflections	16535
<I/σ(I)>	14.48
Completeness [%]	91.1
Redundancy	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	25% PEG 3,350, 50mM Bis-Tris, 2% Glycerol, 100mM BOG