5H1Z
CYP153D17 from Sphingomonas sp. PAMC 26605
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-04-02 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.97933 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 110.837, 110.837, 113.180 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.010 - 3.100 |
| R-factor | 0.26569 |
| Rwork | 0.262 |
| R-free | 0.33956 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.797 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 95.990 |
| High resolution limit [Å] | 3.080 |
| Number of reflections | 14358 |
| <I/σ(I)> | 26.76 |
| Completeness [%] | 91.3 |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 23% (w/v) PEG 3350 |
