5H1X

Crystal Structure of rat Nup62 Coiled-coil motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-12-16
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.98
Spacegroup name	P 1 21 1
Unit cell lengths	30.982, 97.563, 31.502
Unit cell angles	90.00, 112.85, 90.00

Refinement procedure

Resolution	27.825 - 2.410
R-factor	0.2351
Rwork	0.227
R-free	0.30770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3t97
RMSD bond length	0.008
RMSD bond angle	1.120
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHENIX (1.8.4_1496)
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.782	48.782	2.540
High resolution limit [Å]	2.408	7.620	2.410
Rmerge		0.026	0.176
Rmeas	0.047	0.032	0.211
Rpim	0.025	0.017	0.114
Total number of observations	22313	744	3034
Number of reflections	6669
<I/σ(I)>	17.9	38.5	5.9
Completeness [%]	99.4	98.9	98.6
Redundancy	3.3	3.4	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	8	291	100mM Tris-HCl, 50mM Magnesium Chloride, 34% PEG 400