5H1S
Structure of the large subunit of the chloro-ribosome
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TECNAI ARCTICA |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2.7 |
| Detector | FEI FALCON II (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.5 |
| Number of Particle | 338305 |
| Software | EMAN2,RELION,CTFFIND3,PHENIX |
| Symmetry | C1 |
| CTF correction | PHASE FLIPPING ONLY |
