5H1P
CRISPR-associated protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-05-31 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 63 |
| Unit cell lengths | 90.625, 90.625, 50.064 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.664 - 1.750 |
| R-factor | 0.1649 |
| Rwork | 0.163 |
| R-free | 0.19800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5h1o |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.578 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-2000 |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.067 | 0.700 |
| Number of reflections | 23845 | |
| <I/σ(I)> | 8.9 | 3.7 |
| Completeness [%] | 99.0 | 100 |
| Redundancy | 12.3 | 12.3 |
| CC(1/2) | 0.915 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 281 | 100 mM Ammonium acetate, 85 mM Sodium acetate pH 4.6, 23.0% (w/v) PEG4000, 10% (v/v) Glycerol |
